Question Details

Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of:

Options

A

8 bp

B

6 bp

C

4 bp

D

10 bp

Show Answer

Correct Answer :

Option B

6 bp

6 bp

Solution :

The correct option is 6 bp.

Step-by-step Explanation:
1. What is Hind II? Hind II (specifically, HindII) is historically significant as the very first restriction endonuclease (restriction enzyme) to be discovered and isolated from the bacterium Haemophilus influenzae.
2. What is a Recognition Sequence? Restriction endonucleases function by inspecting the length of a DNA sequence. Once they find their specific recognition sequence, they bind to the DNA and cut each of the two strands of the double helix at specific points in their sugar-phosphate backbones.
3. Recognition Sequence Length: It was determined that Hind II always cuts DNA molecules at a specific point by recognizing a sequence of 6 base pairs (bp).
4. Specific Sequence: The specific sequence recognized by Hind II is:
5'-GT(Py)(Pu)AC-3'
where Py stands for a pyrimidine (T or C) and Pu stands for a purine (A or G). Because this sequence consists of exactly six nucleotides, the recognition sequence is 6 bp long.

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