Identify the correct statement.

The correct statement is: RNA polymerase binds with Rho factor to terminate the process of transcription in bacteria.

Let us evaluate each of the given options step-by-step to understand why this statement is correct and why the others are incorrect:

1. Termination of transcription in bacteria (prokaryotes):
In bacteria, transcription is terminated by two main mechanisms: Rho-independent (intrinsic) and Rho-dependent termination. In Rho-dependent termination, a specific protein called the Rho factor (ρ) binds to the growing RNA transcript and moves along it towards the RNA polymerase. Once it catches up, Rho factor interacts with the RNA polymerase, causing it to dissociate from the DNA template, thereby terminating the transcription process. Thus, this statement is accurate.

2. The template versus the coding strand:
During transcription, only one of the two DNA strands is used as a template for mRNA synthesis. This strand is called the template strand (oriented in the 3' → 5' direction). The other strand is called the coding strand (oriented in the 5' → 3' direction), which has the same sequence as the newly synthesized mRNA (except that thymine (T) in DNA is replaced by uracil (U) in RNA). The coding strand itself is not copied to mRNA. Therefore, the statement "The coding strand in a transcription unit is copied to an mRNA" is incorrect.

3. Split gene arrangement:
A split gene arrangement (where coding sequences, exons, are interrupted by non-coding sequences, introns) is a characteristic feature of eukaryotic genomes, not prokaryotic genomes. Prokaryotic genes are typically continuous and lack introns. Thus, the statement "Split gene arrangement is characteristic of prokaryotes" is incorrect.

4. Capping of hnRNA:
During post-transcriptional processing in eukaryotes, capping involves the addition of an unusual nucleotide, methyl guanosine triphosphate, to the 5' end (not the 3' end) of the precursor mRNA (hnRNA). Tailing (polyadenylation) occurs at the 3' end. Therefore, the statement "In capping, methyl guanosine triphosphate is added to the 39 end of hnRNA" is incorrect.