Match List I with List II

List I		List II
A	Lipase	I	Peptide bond
B	Nuclease	II	Ester bond
C	Protease	III	Glycosidic bond
D	Amylase	IV	Phosphodiester bond

Choose the correct answer from the options given below:

The correct answer is A-II, B-IV, C-I, D-III.

To understand the correct matching, let us look at the substrates and the specific chemical bonds targeted by each enzyme listed in List I:

A. Lipase matches with II. Ester bond.
Lipases are enzymes that catalyze the hydrolysis of lipids (fats). Fats, particularly triglycerides, consist of fatty acids bound to a glycerol backbone via ester bonds. Lipases break these ester bonds to release free fatty acids and glycerol.

B. Nuclease matches with IV. Phosphodiester bond.
Nucleases are enzymes that cleave nucleic acids (DNA and RNA). The backbone of DNA and RNA consists of nucleotides linked together by phosphodiester bonds. Nucleases target and hydrolyze these phosphodiester bonds.

C. Protease matches with I. Peptide bond.
Proteases (also known as peptidases or proteolytic enzymes) break down proteins. Proteins are polymers of amino acids linked together by peptide bonds. Proteases catalyze the cleavage of these peptide bonds to break proteins down into smaller peptides or amino acids.

D. Amylase matches with III. Glycosidic bond.
Amylases are enzymes that digest carbohydrates (such as starch and glycogen). Carbohydrates consist of sugar (monosaccharide) subunits joined together by glycosidic bonds. Amylases specifically hydrolyze these glycosidic bonds to break down complex sugars into simpler sugars like maltose and glucose.

By matching each enzyme with the type of bond it hydrolyzes, we get:
A → II
B → IV
C → I
D → III