Regarding catalytic cycle of an enzyme action, select the correct sequential steps :

A. Substrate enzyme complex formation.

B. Free enzyme ready to bind with another substrate.

C. Release of products.

D. Chemical bonds of the substrate broken.

E. Substrate binding to active site.

Choose the correct answer from the options given below :

The catalytic cycle of an enzyme action proceeds through a series of well-defined sequential steps:

1. Substrate binding to the active site (E): First, the substrate molecule binds to the active site of the enzyme.
2. Substrate-enzyme complex formation (A): The binding of the substrate induces the enzyme to alter its shape, fitting more tightly around the substrate to form a temporary substrate-enzyme complex.
3. Chemical bonds of the substrate broken (D): The active site of the enzyme, now in close proximity to the substrate, weakens and breaks the chemical bonds of the substrate, leading to the formation of the enzyme-product complex.
4. Release of products (C): The enzyme releases the newly formed products from its active site.
5. Free enzyme ready to bind with another substrate (B): The free enzyme returns to its original state, ready to bind with another substrate molecule and initiate the cycle again.

Thus, the correct sequence is E → A → D → C → B.