Which of the following is not an application of PCR(Polymerase Chain Reaction) ?

The correct answer is Purification of isolated protein.

Polymerase Chain Reaction (PCR) is a molecular biology technique that specifically amplifies segments of DNA. Its primary uses include:

• Generating large quantities of a specific DNA fragment (gene amplification).
• Detecting mutations or variations in a DNA sequence (detection of gene mutation).
• Providing DNA templates for diagnostic tests (molecular diagnosis).

All of these applications rely on the ability of PCR to create many copies of a nucleic acid sequence, not a protein.

Purification of an isolated protein, on the other hand, involves separating and concentrating a protein from a mixture, typically using methods such as chromatography, electrophoresis, or precipitation. Since PCR does not interact with proteins and does not produce protein products, it cannot be used for protein purification. Therefore, this option is not an application of PCR.