Which one of the following statements is true about Madhubani paintings and the area in which they originated?

Madhubani painting, also known as Mithila painting, is a traditional folk art form that originated in the Mithila region of Bihar (and parts of neighboring Nepal). Traditionally, these paintings were created by women on freshly plastered mud walls and floors of huts, though they are now also done on cloth, hand-made paper, and canvas.

One of the key distinguishing features of Madhubani art is the medium used for painting. The paintings are made using a paste of powdered rice, into which natural colors derived from plants, flowers, and other organic materials are mixed. The colors are vibrant and include black (from soot), yellow (from turmeric), red (from flower extracts), and blue (from indigo).

Let us analyze the given options to confirm the correct statement:
1. "These paintings are done on the floor using brush and indigo in the Mithila region of Bihar." - While they originated in Mithila, they are not exclusively done on the floor, nor do they only use indigo.
2. "These paintings are mainly influenced by the Mughal art and culture." - This is incorrect. Madhubani art is an indigenous folk art style with roots in local religious traditions and mythology, not Mughal art.
3. "It has its roots in the Mithila region of Bihar and these are made out of paste of powdered rice in which colours are mixed." - This statement is completely accurate.
4. "It is an old form of folk art that originated in the State of Rajasthan." - This is incorrect, as it originated in Bihar, not Rajasthan.

Therefore, the correct statement is: It has its roots in the Mithila region of Bihar and these are made out of paste of powdered rice in which colours are mixed.